DNA to Protein Translator

DNA to mRNA to protein, with reading frames and point mutations.

Private by design

Your inputs stay on your device and are not sent to our servers. Optional usage analytics does not receive your files or tool inputs.

DNA to Protein Translator

Transcribe, translate and test point mutations

DNA sequence

Translation

The result appears here.

Options

The sequence I pasted is

The coding strand reads like the mRNA (with T for U). The template strand is its complement; it is reverse-complemented first.

Reading frame

Stop reading at the first stop codon

Three-letter amino acid names

Point mutations (optional)Base, position, new base on the coding strand, counting from 1. Separate several with commas.

DNA is copied into mRNA (transcription: T becomes U), and the mRNA is read three bases at a time (translation). Each codon names an amino acid or a stop. A different starting base gives a different reading frame, so six frames exist: three on each strand. A point mutation can be silent (same amino acid), missense (different amino acid) or nonsense (a new stop).

Real proteins start at ATG (methionine) and end at a stop: TAA, TAG or TGA.
If you only have the template strand, choose Template strand; the tool reverse-complements it.
Use Longest ORF to find the most likely gene in an unknown sequence.
Mutation positions count from base 1 of the coding strand you pasted.

Standard genetic code (NCBI translation table 1). Transcription replaces T with U on the coding strand. Reverse frames translate the reverse complement. ORFs run from ATG to the first in-frame stop. Mutations are classified by comparing the codon before and after the change.

Preparing tool

How to use DNA to Protein Translator

Paste your DNA (plain letters or FASTA) and say whether it is the coding or template strand.
Choose one reading frame, all six frames or the longest ORF.
Add a mutation such as A5G to compare the protein before and after.

Read the detailed guide →

What are the limits of DNA to Protein Translator?

Uses the standard genetic code only (not mitochondrial or bacterial variants) and reads A, C, G and T (U counts as T). It does not look up genes or predict protein function. Your sequence is processed on your device and never uploaded.